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I wouldn't recommend it... Plus from what i've heard and read, the manual in the IS250 isn't a very good one. If you've got your heart set on a manual, you're better off with a different vehicle all together.
Ramon is right. I had my mind set on a MT but was disappointed. Clutch feels spongy. PIck up is good though. Now that there are a lot of complaints about the MT I am so happy I got the 6 spd auto.